Anaerobic bag culture method

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Anaerobic bag culture method.

In a new method of anaerobic culture, a transparent, gas-impermeable bag is used and the anaerobic environment is established with copper sulfate-saturated steel wool. An Alka-Seltzer tablet generates carbon dioxide. The agar plate surface can be inspected through the bag at any time without interrupting the anaerobic atmosphere or disturbing other specimens. Methylene blue indicator strips are...

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A method named "bag culture" was developed for coculturing of Linum persicum (section Syllinum) and L. austriacum (section Linum) hairy roots. For this propose L. austriacum and L. persicum hairy root cultures were established using Agrobacterium rhizogenes in McCown medium. L. persicum hairy roots in bags (1 mm2 mesh) were successfully grown together with L. austriacum hairy roots. The amounts...

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Anaerobic Culture Technique

Peptococcus magnus is a biochemically inactive anaerobic Gram-positive coccus similar in morphology to the staphylococcus. It has been found (Smith, 1975) as part of the normal flora of the mouth, vagina, and skin. Previous reports (Pien et al., 1972; Spaulding et al., 1974) have described significant infections with this organism, but knowledge of its pathogenicity is incomplete since speciati...

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Anaerobic Culture Technique

Peptococcus magnus is a biochemically inactive anaerobic Gram-positive coccus similar in morphology to the staphylococcus. It has been found (Smith, 1975) as part of the normal flora of the mouth, vagina, and skin. Previous reports (Pien et al., 1972; Spaulding et al., 1974) have described significant infections with this organism, but knowledge of its pathogenicity is incomplete since speciati...

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Anaerobic stimulated mixed culture system.

A compartmented, autoclavable culture vessel has been developed for the purpose of studying interactive associations of microorganisms which are essential to the anaerobic decomposition of sewage sludge. The unit employs sterile filter membranes to subdivide the interior culture space into individual compartments. Bacteria cultured in one compartment are denied access to adjacent compartments, ...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1975

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.1.6.527-530.1975